| Registro completo |
| Provedor de dados: |
Scientia Agricola
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| País: |
Brazil
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| Título: |
Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture
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| Autores: |
Pompeu,Georgia Bertoni
Gratão,Priscila Lupino
Vitorello,Victor Alexandre
Azevedo,Ricardo Antunes
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| Data: |
2008-01-01
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| Ano: |
2008
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| Palavras-chave: |
Antioxidant enzymes
Catalase
Nickel
Superoxide dismutase
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| Resumo: |
Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a specific GR isoenzyme appears to respond to the Ni-induced oxidative stress when a much higher Ni concentration was used to induce the stress for the same period of treatment. These results illustrate the importance and advantages of determining individual isoenzyme activities.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015
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| Editor: |
São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
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| Relação: |
10.1590/S0103-90162008000500015
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| Formato: |
text/html
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| Fonte: |
Scientia Agricola v.65 n.5 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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